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Risk management in the testing laboratory
Virágová, Tereza ; Bednářová, Bronislava (referee) ; Vymazal, Tomáš (advisor)
This thesis is dealing with analyzing risks in an accredited testing labotarory. In this thesis will be made a search of available manuals and literature issued for accredited testing laboratory. In this thesis will be conducted selected analyzes for selected processes. The aim of this thesis is to analyze and asses the risks in chosen processes of testing laboratory and make a a simple tools for the management and elimination.
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Mechanisms of iron-sulfur cluster biogenesis in eukaryotes
Temešinko, Tomáš ; Doležal, Pavel (advisor) ; Malych, Ronald (referee)
Many essential cellular proteins use iron-sulfur (Fe-S) clusters as cofactors. These proteins often serve as enzymes, components of the electron-transport chain or as intracellular sensors. Prior to the use of the cluster in a protein, it needs to be formed or created de novo. In total, four different mechanisms of Fe-S cluster biogenesis can be used by the eukaryotic cell - ISC, CIA, SUF and NIF. All of these pathways include a specific targeting system for delivering the cluster to its acceptor protein. Errors in biosynthesis ofFe-Sclustersaremostlylethalandcanleadtofailureindevelopmentofmulticellularorganisms.Despite this a better characterization of these mechanisms is needed as research is currently still in progress. This bachelor's thesis provides current information regarding the mechanisms of Fe-S clusters biogenesis in eukaryotes acquired mostly from mammalian cells, including humans, and from well-known model organisms such as Saccharomyces cerevisiae, Arabidopsis thaliana, and parasitic protist Giardia intestinalis.
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The Bay of Pigs and its influence on U.S.-Cuba relations
Jaroš, Milan ; Pondělíček, Jiří (advisor) ; Perutka, Lukáš (referee)
This bachelor thesis focuses on the Bay of Pigs invasion which had been an important part of the U.S.-Cuba relations in the 1960s. The period between the Cuban revolution and the Cuban missile crisis was marked by rapid deterioration of those relations. The thesis is set exactly in this time frame. The invasion was the outcome of the previous deterioration and Castro's victory became the reason for further escalation of the hostilities between the United States and Cuba. This thesis answers the question what decision-making process led to the actual execution of the invasion, what mistakes caused the invasion to fail, who is to blame for this outcome and what consequences did the American failure have on further evolution of the U.S.-Cuba relations. It analyses steps of the planning process and the influence of all the interested parties on this process. It reaches conclusion that the aggressive approach was implemented because of cold war circumstances and the communist threat, the American fear of losing their influence in the western hemisphere and personal ambitions of involved participants. The responsibility for the failure cannot be assigned to just Kennedy's decision making, the CIA's planning or poorly organized Cuban opposition because the fiasco resulted from all these things together....
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Women in CIA: From Typists to Trailblazers?
Uková, Martina ; Bureš, Oldřich (advisor) ; Plechanovová, Běla (referee)
The diploma thesis Women in CIA: From Typists to Trailblazers? analyzes the role of women in the CIA. The development of female workforce within the CIA is tracked in connection to key reforms of the American intelligence community and demands of liberal feminism. The thesis mainly deals with the change of the role of women in the US society and studies areas of intelligence work where gender discrimination against women took place. Although in the past years the number of women on different positions in the CIA proportionally increased, the Agency top management faces a relative scarcity of women. This lower representation of women in the CIA's leadership can diminish effective accomplishment of the CIA's mission. Key Agency's report served for analysis of the transformation of the status of women in the CIA and also for indentifying concrete problems leading to scarcity of women in the CIA's leadership. The author also proposed some incentives for enhancing female representation in the Agency top management.
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Hledání zpravodajství z lidských zdrojů: Měnící se role HUMINT v rámci americké zpravodajské komunity po 9/11
Lochovský, Jan ; Bureš, Oldřich (advisor) ; Schmidt, Nikola (referee)
Diploma thesis tracks changes of approach to the clandestine human intelligence collection (HUMINT) as part of a wider process of the United States Intelligence Community reform after the September 11 terrorist attacks. Using longitudinal comparative analysis, the author observes which key factors enabling successful HUMINT collection were significantly improved and which were neglected, thus constraining a highly desirable development of this intelligence collection technique so important in combating asymmetric threats such as terrorism. Following factors were identified as critical in improving HUMINT: institutional anchoring of HUMINT, adaptation of operational techniques to successfully monitor decentralized terrorism, 'dependence' of the US intelligence community on technological means of intelligence collection, and availability of qualified and especially linguistically adept intelligence professionals in responsible agencies. Keywords CIA, DIA, HUMINT, intelligence, reform, terrorism, United States of America
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Crisis of U.S. Intelligence? George W. Bush and the decision to invade Iraq
Ondrejka, Miroslav ; Kozák, Kryštof (advisor) ; Anděl, Petr (referee)
The intelligence failure regarding the estimates of the Iraqi regime is often cited as the main reason for President George W. Bush to change the dictatorship of Saddam Hussein. Between 2002 and 2003, the highest figures o George W. Bush's administration often used arguments of the intelligence community to justify their decision to overthrow Saddam's regime by force. Their arguments were based primarily on the information on Hussein's contacts with al-Qaeda and his continuing ability to produce weapons of mass destruction. After the war, these estimates were proven wrong and the intelligence community was marked as the force that triggered the invasion. In my master's thesis called Crisis of U.S. Intelligence? George W. Bush and the decision to invade Iraq, I put forward arguments contradicting this widespread idea. The goal of the thesis is to cast light on the fundaments of the intelligence failure regarding Hussein's alleged contacts with al- Qaeda and his capability to stockpile and produce weapons of mass destruction. I reveal the ideological and strategic reasons behind the Bush's decision to invade Iraq and the fact that the intelligence findings played only secondary role. The true reason of the intelligence failure wasn't only flawed manipulation with human intelligence (HUMINT) and...
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The Bay of Pigs and its influence on U.S.-Cuba relations
Jaroš, Milan ; Pondělíček, Jiří (advisor) ; Perutka, Lukáš (referee)
This bachelor thesis focuses on the Bay of Pigs invasion which had been an important part of the U.S.-Cuba relations in the 1960s. The period between the Cuban revolution and the Cuban missile crisis was marked by rapid deterioration of those relations. The thesis is set exactly in this time frame. The invasion was the outcome of the previous deterioration and Castro's victory became the reason for further escalation of the hostilities between the United States and Cuba. This thesis answers the question what decision-making process led to the actual execution of the invasion, what mistakes caused the invasion to fail, who is to blame for this outcome and what consequences did the American failure have on further evolution of the U.S.-Cuba relations. It analyses steps of the planning process and the influence of all the interested parties on this process. It reaches conclusion that the aggressive approach was implemented because of cold war circumstances and the communist threat, the American fear of losing their influence in the western hemisphere and personal ambitions of involved participants. The responsibility for the failure cannot be assigned to just Kennedy's decision making, the CIA's planning or poorly organized Cuban opposition because the fiasco resulted from all these things together....
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Iron-sulphur cluster synthesis in anaerobic protists
Zelená, Marie ; Hampl, Vladimír (advisor) ; Mach, Jan (referee)
Iron-sulpur clusters are small ubiquitous cofactors of proteins present in all cells. These proteins participate in many important processes such as gene expression or respiratory chain. In vivo synthesis of iron-sulphur clusters requires complex biosynthetic pathways. In eukaryotic cells these pathways are localized inside mitochondria and plastids and in the cytosol. Cytosolic synthesis depends on a product of the mitochondrial pathway. Mitochondria of anaerobic protists in most cases went through reductive evolution, which is associated with the reduction of mitochondrial iron-sulphur cluster biosynthetic pathway. In many cases, these organisms acquired alternative pathways via lateral gene transfer, which act either as a complement to the mitochondrial pathway, or as its complete functional substitution. Replacement of the mitochondrial pathway might have been a key event for secondary loss of mitochondria. Key words: Iron-sulphur clusters, anaerobic protists, ISC, CIA, SUF, mitochondrion
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Export of biomolecules from mitochondria
Pelc, Josef ; Doležal, Pavel (advisor) ; Kovalčíková, Jana (referee)
Mitochondrion is cellular organelle evolved from the endosymbiotic bacteria. Because of its bacterial origin mitochondrion has a number of unique properties and metabolic pathways. Mitochondria or its reduced forms are present in all eukaryotic organisms except a single exception. During the course of evolution most of mitochondrial DNA was transferred to the nucleus. Despite this, mitochondria still encode several proteins that are part of the electron transport chain and the ATP synthase. These proteins are transported from the mitochondrial matrix into the inner mitochondrial membrane. Misfolded proteins of the inner membrane are degraded by mitochondrial proteases, which leads to the release of generated peptides. Fe-S clusters are assembled in the mitochondrial matrix. Still unknown molecule, which is product of ISC pathway is transported into cytosol and participates in the assembly of cytosolic and nuclear iron-sulfur proteins. Other transport pathway ensures the exchange of lipids between the endoplasmic reticulum and mitochondria. Both mitochondrial membranes have different lipid composition. To maintain their properties specific lipid transport is required between the two mitochondrial membranes. This transport pathway is also used for the lipid synthesis in the inner mitochondrial membrane.
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